It’s plot recreation time! In this post, we’ll look at how we can recreate a plot in R. I thought it might be useful to provide the solution but also to detail the process I went through to get there. We have a FACS plot taken from a BD FACS Aria machine: Briefly, it’s a […]

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A detailed walkthrough of recreating a FACS (flow cytometry) histogram plot using R and ggplot2. The author demonstrates the step-by-step process of loading .fcs files with flowCore, transforming fluorescence data to log scale, and experimenting with different geom functions (histogram, step, line, area) to achieve the desired visual appearance. The tutorial covers handling log-scaled histograms, custom color schemes, axis formatting, and exporting both the plot and underlying numerical data for journal publication requirements.

Let It Flow: recreating a FACS plot with ggplot